FastQCFastQC Report
Fri 3 Feb 2017
G98_M1-M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG98_M1-M7.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences143194263
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA229500.034.7257744
ATGCCGA284850.033.32438325
GATCGGA292200.032.888771
CGGTTCA289400.031.79687512
GAGCGGT299400.030.8278059
AGCGGTT299900.030.79187210
ATCGGAA317000.030.7541242
ATCTCGT263800.030.26843843
CGAGACC283500.029.93265229
GCGGTTC310600.029.74604411
AATGCCG321900.029.64448524
TGCCGAG331900.028.69223626
GCGATCT152400.028.46276740
CCGAGAC299500.028.42814628
GAGACCG299750.028.33175530
AGCGATC159600.027.39714639
CGATCTC161400.026.98361241
AAGAGCG363050.026.888027
GCCGAGA350700.026.84351227
TCGGAAG367800.026.5906453
Produced by FastQC (version 0.11.2)