FastQCFastQC Report
Tue 31 May 2016
131127Wax_D13-6260_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6260_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22493961
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10950.018.5529481
GACCGAC10750.017.42879332
GAGCGGT12250.017.230439
TCTCGTA10400.017.17741644
ATCGGAA12400.016.8692742
CGGTTCA12900.016.3622312
ACCGACT12200.015.71448733
CCGACTT13150.015.2419134
ATGCCGA13650.015.00280725
GCGGTTC13800.014.95897311
AGACCGA13400.014.30721831
AGCGGTT14650.014.249372510
AATCTCG14800.012.36502242
ATCTCGT16550.012.2422843
GAGACCG15850.012.09569330
AATGCCG18100.012.03645924
CCGAGAC16900.011.85982928
TCGGAAG18250.011.4600543
AGAGCGG19150.011.3777878
CGAGACC18500.011.06963829
Produced by FastQC (version 0.11.2)