FastQCFastQC Report
Sun 26 Mar 2017
G98_M3M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG98_M3M7.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences59729373
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGCGATCTCGTA795450.1331756822560317Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA130800.036.2918944
ATGCCGA160350.035.88058525
GCGATCT118650.035.1242740
GATCGGA168950.034.7274171
AGCGATC123000.034.05918539
CGATCTC123100.033.8191541
GAGCGGT168100.033.633769
CGGTTCA167200.033.55116312
AGCGGTT169000.033.50955610
GCGGTTC170050.033.17985511
CGAGACC157650.033.05424529
AATGCCG175000.033.00135424
ATCGGAA179200.032.9869652
GATCTCG130950.032.9397142
ATCTCGT144150.032.74947443
GACCGGC142250.032.66625632
CCGAGAC162500.032.13473528
TGCCGAG178750.032.08964526
AGACCGG147100.031.84093731
GAGACCG165850.031.60386830
Produced by FastQC (version 0.11.2)