FastQCFastQC Report
Wed 12 Jul 2017
G97-M8_AGTTCC_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M8_AGTTCC_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23221689
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG38300.024.84857241
GTTCCGT45550.021.49497835
CTCGTAT45400.020.95971944
TTCCGTA47350.020.74329636
CCGTATC48300.020.08294938
TCCGTAT50250.019.84972637
ACGATCA46350.019.6750812
CGTATCT49000.019.67175739
CACGATC52100.018.9115561
GATCGGA50450.018.5626371
ATCTCGT52550.018.34232542
TATGCCG54250.018.1029948
CGATCAC50850.017.6938933
ACGTCTG61800.016.3361115
TCGTATG62800.015.54130145
TCTCGTA65500.015.17969443
CGTATGC64400.015.01290646
ACACGTC70300.014.75033613
AGTTCCG70800.014.08750134
ATCGGAA67900.013.8349012
Produced by FastQC (version 0.11.2)