FastQCFastQC Report
Wed 12 Jul 2017
G97-M4_GCCAAT_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M4_GCCAAT_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17018238
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG30400.013.3452539
ACGATCA28100.012.5907562
CGATCAC28850.012.5805243
CGCGAAT14550.012.5789551
CACGATC32700.011.940391
TATGCCG40100.011.8652753
CGCGGAT12400.011.8079881
CGCCAAT36900.011.32428933
CTCGTAT35850.011.316174542
GTCACGC37800.011.13528829
CGATACT21500.011.06489861
CCACTAG42300.011.03185753
CTCGAAT35300.010.8016871
CGCGTAT3151.9440533E-510.6521791
CGCCGTT7000.010.4585031
ATTAACG22550.010.41464120
TAAGGCG31700.010.3905649
CCGGAAT33850.010.2731231
AATCGTG14100.010.1664655
CGTATGC48150.010.13631251
Produced by FastQC (version 0.11.2)