FastQCFastQC Report
Wed 12 Jul 2017
G97-M3_ACAGTG_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M3_ACAGTG_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19918376
Sequences flagged as poor quality0
Sequence length67
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCT227380.11415589303063664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA62400.027.7621562
CACGATC66750.026.7339251
CGATCAC76900.022.48773
CTCGTAT44700.019.9259642
GATCTCG49050.018.0350439
ATCTCGT50100.017.8395440
TATGCCG51250.017.55598446
GATCGGA49800.016.293351
CGTATGC60700.015.37501744
TCGTATG63050.014.94707843
TCACAAG125700.014.678526
TCTCGTA62350.014.62811941
ACGTCTG60250.014.42765615
ATGCCGT62800.014.37570447
GCCGTCT65150.014.18490249
CGCCATT34750.013.7817051
AGTGATC66700.013.76547336
ATTAACG80850.013.73147920
TAACGAA83450.013.30348922
TTAACGA84900.013.07680421
Produced by FastQC (version 0.11.2)