FastQCFastQC Report
Wed 12 Jul 2017
G97-M2_TGACCA_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M2_TGACCA_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17285669
Sequences flagged as poor quality0
Sequence length67
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA45600.021.9384062
CACGATC49500.021.1993941
CGATCAC52800.019.3509333
CGCGTAT2653.6379788E-1117.2669491
TATGCCG32450.013.2524283
CTCGAAT29850.013.183041
ATTAACG51850.012.94061520
TCACAAG94000.011.7121466
TAACGAA57250.011.61360422
TTAACGA57400.011.53032121
ATTGGAC79950.011.2528016
CGCGTGT9650.010.7478541
TAAGCCG15950.010.70834146
ATGCCGT43900.010.55987554
GCCGTCT47100.010.42466456
ATACGTC57350.010.42392431
CGAAAAG65400.010.35321125
AATTGGA99450.010.3349865
CGTATGC42000.010.3135771
CATACGT61250.010.30796430
Produced by FastQC (version 0.11.2)