FastQCFastQC Report
Wed 12 Jul 2017
G97-M10_CCGTCC_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M10_CCGTCC_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16128457
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC27250.020.70627632
CGTCCCG27600.020.002635
GATCGGA29450.018.2304291
CTCGTAT31850.017.8110544
CCGATCT33450.016.77986539
TATGCCG40950.016.7580723
GATCTCG34700.016.52694141
CACCCGT35600.016.10657731
ATCTCGT35800.015.59270242
CCCGATC35700.015.46582538
ACGTCTG37600.015.25022315
TCACCCG45150.014.65876630
TCCCGAT38350.014.63548637
CGTATGC48350.014.3218411
GTCCCGA40100.014.14896336
CCGTCCC41400.013.92420434
ATGCCGT53150.013.1406524
CCCGTCC45100.012.71423333
ATCGGAA43700.012.1441552
CTCGAAT35500.012.1160271
Produced by FastQC (version 0.11.2)