FastQCFastQC Report
Thu 23 Jul 2015
G96-M6_GATCAG_L012_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M6_GATCAG_L012_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4795609
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG9400.014.343974
CTCGAAT13800.013.2436651
CCGATTA9850.012.7448813
CTCCGAT17050.012.6431741
CGCGAAT5202.1105734E-812.6167471
CGACAAC11200.012.5523751
CGCCAAT7157.2759576E-1212.4528911
CGACAAT6201.540684E-912.0934711
TCGCGTG3106.0580776E-412.0910572
TCCGATT11850.011.8614792
TAGCCTC21750.011.632432
TCGGTTA6051.3898898E-811.6164072
CGCTCGT3258.992308E-411.5353111
CTTAGAT17950.011.4871131
CTAGCCT24300.011.3780641
CTCGCCG6352.831257E-811.0698111
CTCGTTT14050.011.006781
CGCCATT11150.010.9275081
CGTAGCT8301.1459633E-1010.7274911
GCGGAAT7051.1545126E-810.6353931
Produced by FastQC (version 0.11.2)