FastQCFastQC Report
Thu 23 Jul 2015
G96-M5_ACTTGA_L012_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M5_ACTTGA_L012_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12729787
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG43700.011.4116193
CGCGAAT14950.011.1040291
CTCGAAT34450.010.8421411
CGATTAG19050.010.5352184
CGCGGAT11001.8189894E-1210.3753281
CGCCAAT16800.010.1900531
GTTCGAT19950.010.1412981
CGCGCAT9803.7471182E-1010.0577141
CACTAGC48150.010.0462854
ACTAGCC45900.09.9778665
CGATCAC19750.09.3711383
TAAGGCG40350.08.9517739
CGGCAAT25050.08.9049721
TCCGATT27450.08.8822992
CGCGGTT12302.6921043E-108.8569871
CCGGAAT39150.08.7454871
CCGATTA20650.08.7137463
CGAAAAT25800.08.6461061
TTCGATT21100.08.6050912
TAGCCTC55050.08.6042127
Produced by FastQC (version 0.11.2)