FastQCFastQC Report
Thu 23 Jul 2015
G96-M5_ACTTGA_L012_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M5_ACTTGA_L012_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12729787
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT34200.013.4333691
CGATCAC19650.011.5951773
CGCGCAT8903.6379788E-1211.0615061
CGCAATT17750.010.8285541
CCGGAAT39950.010.7958311
GTCGATT11850.010.6814541
CGCGGAT11250.010.4177161
CGATTAG18500.010.3049874
CGCCGTT9105.638867E-1110.3032351
CGCTCGT10550.010.2202231
CGCGTGT9752.0008883E-1110.0971711
CGACAAT13800.09.8515341
CCGGCAT30450.09.6992521
CCGAAAT21350.09.6613931
ACTAGCC48400.09.6070115
CACGATC32400.09.5495721
CCACTAG45900.09.5226773
CTCGTTT32550.09.5055651
CCGGGAT29300.09.2799441
CGCATAT6102.418123E-59.22223951
Produced by FastQC (version 0.11.2)