FastQCFastQC Report
Thu 23 Jul 2015
G96-M2_GTCCGC_L012_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M2_GTCCGC_L012_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10213958
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA28400.025.4066752
CGATCAC30600.024.4637093
CACGATC33150.022.4767761
CTCGAAT27300.013.7324441
CGCTTAT4303.6746496E-713.0777571
CTCGATT8500.012.6802951
CGCCATT24450.012.6498551
TCACAAG71350.011.8591276
CGCGTGT8055.456968E-1211.6427241
CGGCGAT8157.2759576E-1211.4998671
CGACCTA13950.010.6641868
CGGGGAT20500.010.51536751
CGATTGG71000.010.4776164
CCACGAT35350.010.4726871
CCGGAAT33800.010.3983641
ATTGGAC75700.010.2564236
AGTCTAA66200.010.1832256
CGCGAAT13450.010.1040661
CGAATGT35000.010.0963263
GAATCGC14950.09.9523733
Produced by FastQC (version 0.11.2)