FastQCFastQC Report
Thu 23 Jul 2015
G96-M1_CCGTCC_L012_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M1_CCGTCC_L012_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8717311
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC20200.028.7942333
ACGATCA20900.026.3261832
CACGATC25650.022.2607271
CGCCATT18900.016.2040751
CGTATAC3507.6216186E-411.7652283
CCGGGAT20250.011.5351051
AAGACGT45850.011.3386273
CGCGTTA5002.007965E-510.6208788
TCACAAG54150.010.4676666
CGCCAAT11500.010.3815951
GAATCGC15000.010.2945753
CGAGGAT22200.010.0542461
CGCGTAG4150.002941592410.0063551
CGGGGTT21800.010.0006181
AGACGTG51300.09.9427944
ACGTGGT48350.09.9115616
CGCAAAT7356.145183E-79.8872321
CGGGTTT14350.09.7666561
TCGGTTT22200.09.5864812
CGGGGAT21200.09.5491081
Produced by FastQC (version 0.11.2)