FastQCFastQC Report
Fri 10 Jun 2016
G95-M8_AGTTCC_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M8_AGTTCC_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7689233
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAGC8402.5102054E-1011.1735966
CTCGCGT4809.640706E-510.5429961
CCGGGAT19500.09.8617561
CGCAAAC9553.910827E-99.5383761
GCGGAAT7501.1450138E-69.4465251
CGCGGGT7004.867383E-69.3983281
CGGCAAT8759.962059E-89.2537391
CGGGAAT13001.4551915E-118.9534371
CGGGCAT22850.08.4159411
CGCCAAT10853.4673576E-88.3955291
CCGAAAT14552.0008883E-118.3474451
CGACAAT7451.0276113E-48.1513641
CGCACAT12505.3842086E-98.097021
CGGGTAT7551.20005425E-48.0433981
ATTGGAC28550.08.03611856
CGGGGTT17101.8189894E-127.99048141
CCGGGGT23500.07.96781351
CCGGGTT19700.07.9634411
GCGAGTT10853.0922638E-77.92911051
CCGACAT14107.585186E-107.89603141
Produced by FastQC (version 0.11.2)