FastQCFastQC Report
Fri 10 Jun 2016
G95-M8_AGTTCC_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M8_AGTTCC_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7811192
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT16850.011.773281
CCGGCTT17450.011.6391471
CGCCGTT5454.7570757E-711.2666541
CGCGAGT4651.145062E-511.1734641
CTCGATT7652.764864E-1011.1137121
CCGGGAT20650.010.9791631
CGCGAAT3903.1461412E-410.8999871
CTCGTAT13050.010.68584544-45
CGTATGC14150.010.67640646-47
CCGAAAC13750.010.6489581
CCGCAAT8451.382432E-1010.6205011
CTCGAAT12050.010.5833911
CGTACGT4156.4692856E-410.0806147
CGTCAAT8451.5552359E-910.0615271
CGGCGAA5203.9358667E-59.9916551
CCCGCAT14900.09.8270581
ATATACG4752.045311E-49.78667456
CCGGCAT16000.09.7418641
CGACGAT4852.148766E-49.738821
CGGAAAT11650.09.7304611
Produced by FastQC (version 0.11.2)