FastQCFastQC Report
Fri 10 Jun 2016
G95-M7_AGTCAA_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M7_AGTCAA_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10135439
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAT8505.875336E-1010.6432891
CGCGCAT5604.4905544E-59.8724961
TCGCGTA3552.0709922E-79.66356968-69
CGGGTAT9453.6725396E-99.5733291
CGACGTT5806.59681E-59.5320641
CGGAAAT18250.09.0881041
CGCAGTT14700.08.889521
CGCAATT4550.00819143658.8369191
CGCACAT15450.08.7832971
CCGGAAT24400.08.6513071
AAATCGA14751.6370905E-118.4257344
CTCGAAT13856.91216E-118.3464211
CGACAAT10954.5525667E-88.2619131
CGCCACT25800.08.1818561
CGCGGTT9856.75047E-78.1640571
CCGCGTT5550.0044793638.1502661
CGGGGAT26200.08.0569431
CCGGGAT26250.08.0415961
CGTCGAT9403.0344363E-68.0202091
CGCCGAT5650.00526001778.0060141
Produced by FastQC (version 0.11.2)