FastQCFastQC Report
Fri 10 Jun 2016
G95-M7_AGTCAA_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M7_AGTCAA_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10135439
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1750.002499316115.9461091
CGCGCAT4855.7480065E-1014.3843771
CCGGCAT18550.012.7869741
CCGGAAT20400.011.8553581
CCGCGAT6755.293259E-1011.7134991
CCCGTAT6655.2204996E-911.1902521
CGCGTTT5557.3438787E-710.8941141
CGCAATC6102.1489359E-710.6737282
CGGGTAT9257.2759576E-1210.558911
CAATCGA7252.0110747E-810.26324
CGACAAT10501.8189894E-1210.1877921
CGCCGTT6454.6224523E-710.0950821
CGCCGAT5558.669029E-610.0561051
CCGCAAT10205.456968E-1210.0314571
CGCGGAT6152.742081E-69.8312731
CCGAAAT18150.09.7375231
CTCGACT17350.09.6503831
CGACGAT4359.911754E-49.6226521
CCGGGAT23350.09.5608371
CGCGTAC4500.00135128779.3018971
Produced by FastQC (version 0.11.2)