FastQCFastQC Report
Fri 10 Jun 2016
G95-M6_CTTGTA_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M6_CTTGTA_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7812714
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1750.002499829715.9455521
CCCGTAT7451.8189894E-1212.4853321
CGGAAAT11850.011.7741421
ACGATCA11050.010.9426762
CCGGGAT16400.010.7762121
TCGAATC8201.05501385E-1010.77586652
CGATCAC11650.010.3791053
CGCATAT5406.2567397E-610.335081
CTCGCGT4551.319068E-410.2215081
CGGCGTT6454.6240712E-710.0947291
CGAAAAT8452.0336302E-99.9070011
CGCGCAT4258.011023E-49.8487241
ATACGCT5204.6730136E-59.8366916
CGCTTAT7101.625067E-79.82560351
CCGCTAT8059.686119E-99.8215361
CGAATTG5801.4638235E-59.6220073
CTCGGAT19850.09.6061581
TTCGCTA7352.689576E-79.4910972
CTCGATT9351.1586963E-99.4507951
CGCCACT20750.09.4136391
Produced by FastQC (version 0.11.2)