FastQCFastQC Report
Fri 10 Jun 2016
G95-M6_CTTGTA_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M6_CTTGTA_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7912602
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAT7502.3646862E-1112.7560771
CCCGAAT13350.011.5457641
CGCAAAC10103.8198777E-1110.52481651
CGCATAT5201.3193878E-410.2212161
TACCGTC5203.6387555E-510.0632389
CGACGAT4250.002945389610.0047661
ATCGAAC8203.4906407E-89.9045536
CCGGGAT17400.09.7747721
CCGGAAT17700.09.6090981
CGCCAAT10154.798494E-99.4256731
GTCGACT7901.1913289E-69.4190441
CGACACT10701.1859811E-88.9411751
CTCGAAC19750.08.8808141
CTTGGAT62200.08.4596181
CCCGTAT6309.022079E-48.4365591
ATTGACG9754.973026E-78.3299836
AAATCGA13452.728484E-108.3005944
CGCCTAT8352.430592E-58.27491
ATCGGAC8052.7350026E-58.1974026
CTCGGCT24800.08.1440011
Produced by FastQC (version 0.11.2)