FastQCFastQC Report
Fri 10 Jun 2016
G95-M5_CAGATC_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M5_CAGATC_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4823792
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT3100.00324873611.9998271
CGCCAAT5802.3126086E-711.9111581
CTCGGTT10305.4569682E-1110.3188511
CCCGAGT14400.09.5950991
CTCGCAT5602.7876362E-49.4896591
CTCGAAT8452.9363764E-79.4335071
GGAATCG7004.6857676E-69.4262283
CTCGAGT10205.2314135E-99.37801551
CGGACTT6852.162394E-59.3095631
CGGTATC5803.9637904E-49.1622062
CGGAAAT8151.822611E-69.1287021
CTTGGAT36800.09.0976941
CCGGTAT6501.2441882E-48.9932771
GTTCGTT5400.0021107788.8570161
CGAGGAT10205.208858E-88.8570151
CGAACCT6351.5902315E-48.7924843
CGAGAAT13359.094947E-118.7574981
CGCCATT12753.3833203E-108.7528151
AATCGAG5806.27715E-48.7499215
GTTCGAT6106.555815E-48.7118181
Produced by FastQC (version 0.11.2)