FastQCFastQC Report
Fri 10 Jun 2016
G95-M5_CAGATC_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M5_CAGATC_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4823792
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT11200.016.0232411
CGATCAC6956.730261E-1112.0404593
ACGATCA6603.6689016E-911.4444832
CTTCGAT8601.6370905E-1110.98288251
GTCGCAT3500.001566038710.7946031
CTCGATA3953.5438145E-410.7604451
CGCATAT3550.001762530510.6425691
CCGACAT8903.274181E-1110.6126731
GTCGGAT4902.0476595E-510.6018431
CTCGGTT9901.8189894E-1210.4947551
CTCAGAT44500.010.2942921
CGGCTAT4155.589557E-410.2418681
GCGATCG3650.002530566910.1894893
CCGGACT7603.2892785E-89.9423991
CACGAAC7301.9472282E-79.7040531
CGGGGTT9852.2191671E-109.5891151
CGGAGAT9852.2191671E-109.5891151
CCCGGAT10901.6370905E-119.5319331
CCGCCAT11555.456968E-129.404391
CTCGAAT8159.6701115E-89.2714391
Produced by FastQC (version 0.11.2)