FastQCFastQC Report
Fri 10 Jun 2016
G95-M4_GCCAAT_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M4_GCCAAT_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7019152
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1350.006752030518.633611
CGCCAAT9350.013.9901531
CGCCGTT4554.451198E-612.1630381
CGATCAC11200.012.0092633
CGCAAAC7254.6929927E-1011.7970031
CCGGAAT15650.011.2516041
ACGATCA9907.2759576E-1210.6717252
CGCATAC5704.667607E-610.5917361
CGCGTTT4000.00280899910.062151
CCGGGAT17000.09.7662041
GTCGGAT7852.232282E-79.6135191
CGGGATT11106.366463E-119.51824951
CACGATC14000.09.3434241
CGCAAAT5958.623384E-59.3011461
CGCGGTT6009.447854E-59.2236381
CCGGAAC16050.09.0904151
CGAACTC17650.09.0861113
CTCGAAT12254.7293724E-119.03539851
CGTAACC4600.0070702228.9969063
CGTGAAT10251.454282E-88.8350581
Produced by FastQC (version 0.11.2)