FastQCFastQC Report
Fri 10 Jun 2016
G95-M4_GCCAAT_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M4_GCCAAT_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7019152
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG1900.00432359514.68608955
ACGATCA9500.013.7074072
CGATCAC10400.012.0740033
CGCATAC6705.86806E-911.1066891
CGCCTAT7552.8740033E-1011.0883011
CCCGAAT11900.010.9433561
CCGGAAT15000.010.54211
CCGGCAT12850.010.4962551
CGCATAT5406.254195E-610.3353921
CGCGAAT3150.0098753710.3353911
TAAATCG4207.186936E-49.9658453
CGCGAGT4258.0085965E-49.849021
CCGCATT8151.1847078E-89.7013191
CGCACTT9601.8189894E-109.689431
CCGGGAT17350.09.3822721
CGGGTAT5651.1553847E-49.05491
CTCGGAT18100.08.9935041
CGCCCAT13450.08.9906381
CGCGCTT5205.044249E-48.9440891
CCGGAAC16650.08.9387171
Produced by FastQC (version 0.11.2)