FastQCFastQC Report
Fri 10 Jun 2016
G95-M3_ACAGTG_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M3_ACAGTG_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8852061
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT7653.45608E-1112.5011891
CCGGAAT19750.011.836571
CGAAAAT10450.011.6936961
GTCGGAT10053.6379788E-1211.1018021
CGGATCG4707.5989505E-510.7932553
CGCGTGT9056.6393113E-1010.5673041
CGCACGT7404.903213E-710.0516321
CGCCAAT11757.2759576E-129.9477551
CCGGGAT19900.09.8784521
CGACAAT9209.751602E-99.817511
CGGGAAT16350.09.7486341
ACGATCA10901.6189006E-109.7483112
CGGATAT11908.731149E-119.3758921
CGCAAAT7405.3141794E-69.3336581
CGACGAT5200.00150058519.1955861
TCGATAC6401.0542202E-49.1314262
CGATCAC11751.6916601E-109.0663343
TCGGATC15101.8189894E-128.7960752
CGCGGTT6651.5986334E-48.788431
CGACCGT4850.0087685298.76372051
Produced by FastQC (version 0.11.2)