FastQCFastQC Report
Fri 10 Jun 2016
G95-M2_TGACCA_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M2_TGACCA_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6140310
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT9700.013.1684111
CGACGAT3505.755023E-412.1651041
CGCAAAT5501.5476799E-611.6121441
CGCCATT15000.011.3540961
CGAGTAT5602.3902548E-510.4543861
ACGATCA7707.412382E-810.3677872
CCGATAT5753.1987118E-510.1816631
CCGGCAT11553.6379788E-1210.1375871
CTGGAAT66800.010.1186161
CCGGAAT14050.09.8489721
CGATCAC8304.0850864E-89.8042693
CCGGATT9501.6007107E-89.5239951
CGGGAAT10104.305548E-99.48516751
CGCAAGT7405.197129E-69.3498691
CCCGGAT14350.09.2721821
CTCGAAT10951.7589628E-99.2349241
CGCCGGT5250.00161146939.1238281
CGAAGAT14001.8189894E-129.1238281
CGGATAT8201.9406089E-69.0867391
CGCCGAC4700.00668105069.059121
Produced by FastQC (version 0.11.2)