FastQCFastQC Report
Fri 10 Jun 2016
G95-M2_TGACCA_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M2_TGACCA_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6140310
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATT3306.6508495E-512.8793031
CTCGAAT10250.011.97881
CGAAAAT5706.807204E-811.5989041
ATCGACG4358.399848E-510.6871576
CCCGGTT11550.010.6305361
CGCCTAT6705.565562E-810.5725621
CCGCAAT5557.2281255E-610.2106191
CTCGGAT17000.010.0004011
CGAATGT10307.2759576E-129.9297983
CGCAAAC8159.229552E-99.8504281
CTGCGAT14600.09.7035841
CGCGGAT3900.00384697749.6869971
CCGGAAT13650.09.6869971
CTCGTTT12900.09.5180381
CCGGGAT14950.09.476411
CCCGTAT6001.823887E-59.4448221
CTCGGTT11605.456968E-129.3634011
TCGAATC7151.5381811E-69.2436972
CCGGAAC13800.09.23951
TCGGATC10254.765752E-109.2114892
Produced by FastQC (version 0.11.2)