FastQCFastQC Report
Fri 10 Jun 2016
G95-M1_CGATGT_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M1_CGATGT_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7437049
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT4251.6752407E-512.5150731
CGCAAAC8953.6379788E-1211.8858231
CGCGTGT6901.4886609E-811.5628391
CGGCGAT6609.9867975E-811.2825271
CGATCAC10450.011.1847733
CGAAAAT9551.4733814E-1010.5821161
CGACAAC12900.010.3079571
CTCGAAT12400.010.2946561
CCGCGTT4655.33163E-410.2946561
CCGGAAT16800.09.8146471
CGCCACT17400.09.7818961
CGTATAG4200.00387391639.6788445
CTCGGAT17650.09.6433421
CTCGCGT5000.00103810599.5740311
ACGATCT10151.029548E-99.5126873
CCGGCGT8553.4398E-79.3314131
CGGAATC14450.09.2018962
GTCGAGT6753.0721887E-59.0366586
CCGATAT7103.2664455E-58.9896991
GTCGAAG15550.08.8933471
Produced by FastQC (version 0.11.2)