FastQCFastQC Report
Fri 10 Jun 2016
G95-M1_CGATGT_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M1_CGATGT_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7437049
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT8750.015.6544111
CGCGCAT3901.6050635E-613.3221681
CGCAAAT5806.5992936E-912.2154681
CCGGAAT13850.011.9361731
CTCGCAT9450.011.4958971
CGCCATT14950.011.3738661
CGACAAT8751.8189894E-1211.3359531
CCGGATT9600.011.3162741
CTCGAAT12950.011.3067751
CGCACGT4751.4497244E-510.9382011
CCGGCAT15200.010.8760521
CCGGGAT18750.010.8321331
CGACGAT4003.9759063E-410.6274571
CTCGGAT19450.010.4422881
CGTCGAT5153.539235E-510.0886331
CGCCCTT14450.09.8061881
CTCCGAT14950.09.7941631
CGCAGAT19050.09.6697771
CGGGATT12300.09.6002321
CTCGGTT14000.09.4466291
Produced by FastQC (version 0.11.2)