FastQCFastQC Report
Tue 11 Oct 2016
130729Wax_D13-3615_NA_sequence_combined_seq_runs.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130729Wax_D13-3615_NA_sequence_combined_seq_runs.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences28811735
Sequences flagged as poor quality0
Sequence length50-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT52450.047.19675445
TCTCGTA67400.037.2792544
ATGCCGA75150.035.8628525
GATCTCG70700.035.70067642
AGACCGC72450.034.94500431
GATCGGA79400.034.8091931
ATCTCGT72050.034.78543543
CGAGACC73600.034.67265729
GACCGCC74800.033.96766332
CGGTTCA80650.033.8622912
ATCGGAA83600.033.147932
AGCGGTT83050.032.9914310
CGCCTGA76450.032.9655935
GAGCGGT84450.032.6298839
GAGACCG78350.032.5706730
CCGAGAC78200.032.4897928
GCGGTTC84600.032.33422511
AATGCCG83900.032.28270724
ACCGCCT78750.032.2206833
AAGAGCG90400.030.6797397
Produced by FastQC (version 0.11.2)