FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2468_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2468_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20939769
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATT24600.027.30635833
GACCGAT24450.027.2652232
GATCGGA28400.027.245051
ATGCCGA28000.026.71284125
CGGTTCA28950.026.48095912
CCGATTC25450.026.26065434
GAGCGGT29200.026.2541779
AGCGGTT29450.025.97358710
ATCGGAA30600.025.3344442
AGACCGA27400.024.76419331
CGAGACC28700.024.2278929
GCGGTTC32050.024.13170811
CCGAGAC29100.024.06846228
AAGAGCG33150.023.3822357
TGCCGAG32650.022.8042826
AATGCCG33650.022.3286624
AGAGCGG35250.021.8928078
GAGACCG31650.021.8676330
TCGGAAG36600.021.3182143
CGGAAGA37100.021.3056684
Produced by FastQC (version 0.11.2)