FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2467_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2467_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19365843
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGG374860.19356761283255267Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA39550.030.83048232
GATCGGA46650.029.9281671
ACCGTAG41100.029.79190833
ATGCCGA46650.029.51582125
CGGTTCA48700.028.83096112
ATCGGAA49800.028.265612
CGAGACC46950.028.1363329
AGACCGT43850.028.00111431
CCGTAGG43800.027.91630434
AGCGGTT50200.027.83374410
GAGCGGT50250.027.8058349
GCGGTTC50550.027.77553411
TGCCGAG50000.027.47026626
AATGCCG50850.027.14493624
CCGAGAC49000.027.06098228
GAGACCG49250.026.72499830
AAGAGCG54000.026.0322637
TCGGAAG54050.025.9776733
GCCGAGA53850.025.28589827
AGAGCGG56600.024.8964988
Produced by FastQC (version 0.11.2)