FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2460_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2460_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24757647
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCC2773131.1201104854593007Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT370450.033.2348949
CGGTTCA369800.033.1325612
AGCGGTT372250.033.01482410
GCGGTTC372500.032.90596411
AAGAGCG377850.032.8539247
AGAGCGG377500.032.798838
GACCGGT303250.032.71582432
ACCGGTC303200.032.7043933
CGGAAGA380400.032.689034
AGACCGG334650.032.51214631
GAGACCG333300.032.4683930
CCGGTCC308800.032.39203334
CGAGACC334050.032.3736829
CCGAGAC333950.032.36020328
ATGCCGA369500.032.30036525
GCCGAGA364700.032.0545527
TGCCGAG372250.031.96584926
AATGCCG376450.031.96149424
GATCGGA401100.031.0515651
ATCGGAA404350.030.8193132
Produced by FastQC (version 0.11.2)