FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2455_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2455_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21418624
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTTATATCTCGTA601900.2810171185599971Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA71800.041.57248735
ACCGCGT73500.040.46291733
GCGTTAT74300.040.2035736
TCTCGTA74100.040.19887544
CGTTATA74850.039.9942637
TATCTCG77000.038.9963542
ATGCCGA86350.038.44405425
GACCGCG78750.038.1017432
CCGCGTT78750.038.0721234
ATCTCGT79050.037.6251443
AGACCGC80150.037.3544831
AATGCCG92450.036.2387324
CGAGACC83500.036.17239429
GAGACCG83750.036.1173730
CCGAGAC83850.035.99853528
GATCGGA94700.035.96451
TGCCGAG93100.035.7300726
ATCGGAA96650.035.4542662
CGGTTCA97300.034.7204512
GAGCGGT97700.034.601469
Produced by FastQC (version 0.11.2)