FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2453_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2453_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22471549
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA12600.019.72961644
GATCGGA14350.018.3983441
ATGCCGA18250.015.06757625
GACCGAA15300.014.95280532
ATCGGAA18800.014.8577222
GCGGTTC19250.014.39656111
AGCGGTT19200.014.20490710
ATCTCGT17850.014.04975243
CGGTTCA20000.013.85665812
GAGCGGT19700.013.8445619
AATGCCG21800.012.41181224
AGACCGA19150.012.29135131
CGAGACC20950.012.07547629
ACCGAAG20600.011.85298733
AAGAGCG25150.011.6313647
CCGAGAC22850.011.55398728
GAGACCG21800.011.50388630
TGCCGAG25000.011.35221926
TCGGAAG26650.010.9765673
CGAAGCT21250.010.97213135
Produced by FastQC (version 0.11.2)