FastQCFastQC Report
Sun 26 Mar 2017
G91_M1-M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG91_M1-M4.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences99894788
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA228200.036.5833944
ATGCCGA263400.036.08724225
GATCGGA267500.035.79821
CGGTTCA278250.034.62619812
ATCGGAA285650.034.413112
GAGCGGT286000.033.942889
CGAGACC257050.033.62085329
AGCGGTT288500.033.58013510
AATGCCG289300.033.09004624
CCGAGAC265850.032.95074528
ATCTCGT253750.032.93937743
GCGGTTC294450.032.86314811
TGCCGAG291050.032.4786826
GAGACCG264950.032.3757130
CGCATCA129450.031.94788235
AGACCGC164150.031.68375231
AAGAGCG312350.031.500747
GCCGAGA300600.031.02969727
ACCGCAT134550.030.94998233
TCGGAAG322600.030.6010193
Produced by FastQC (version 0.11.2)