FastQCFastQC Report
Sun 26 Mar 2017
130524Wax_D13-2447_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2447_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22717528
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGA38000.033.9237625
GATCGGA37800.033.5789641
TCTCGTA32750.033.24735644
CGGTTCA40000.032.3837212
ATCGGAA42250.031.3864352
GAGCGGT42300.031.0399979
TATCTCG30700.030.8064542
AGCGGTT42550.030.80593510
AATGCCG43900.029.46267724
AGACCGT30850.029.31019631
GCGGTTC44550.029.27375411
CGAGACC39350.028.95543529
CCGAGAC40150.028.76597228
TGCCGAG45150.028.45511626
GAGACCG40050.028.0705230
ACCGTGC32050.027.9356233
ATCTCGT39500.027.89715243
AAGAGCG48900.027.4339667
GACCGTG33450.026.96377232
GCCGAGA46600.026.86158627
Produced by FastQC (version 0.11.2)