FastQCFastQC Report
Wed 22 Jul 2015
G88-M9_ATGTCA_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M9_ATGTCA_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13401558
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT34450.013.659431
GAATCGC19800.011.9093223
CGGAAAT30000.011.8880161
CGACGAT9250.011.7809171
CTAGCCT63350.011.7285091
CGACGGT8709.094947E-1211.3869891
TAGCCTC61050.011.1945192
CGAATCG4153.8863617E-410.6538223
CGCGCAT9001.8371793E-1010.4570511
CTGGAAT180050.010.3991261
ATTGGAC53850.010.2174686
GTTCGAT20900.010.1910841
CTCGTTT31700.010.1566911
CCCGAAT30450.010.0856191
GATCGGA82500.010.072288592-93
CTTAGAT43800.010.0650061
CGATTAG20050.09.800694
AGATCGG97350.09.77869292-93
CCGGAAT40750.09.724351
CGCGGAT10302.0918378E-109.6181351
Produced by FastQC (version 0.11.2)