FastQCFastQC Report
Wed 22 Jul 2015
G88-M9_ATGTCA_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M9_ATGTCA_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13401558
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC18650.011.9644083
CGGGAAT25900.011.6744771
CGATCAC20750.011.6496823
TAAGGCG56600.011.4977079
CGATTCG3752.5359367E-411.1568113
ACGATCA21650.010.7359612
CGGAAAT30050.010.6814061
CTAGCCT63250.010.4436341
CGCGTGT10700.010.43401051
CACGATC29000.010.2661071
CTCGAAT37500.010.1720011
CCGGAAT41650.010.1636621
AGATCGG96700.010.1439492-93
TCGTTTT38350.010.0610082
ATTCACG15400.09.9608436
CTTAGAT44400.09.9532391
CGCAAAT11800.09.8555711
CGAAAAT28150.09.7498391
GATCGGA83750.09.71412992-93
TAGCCTC59500.09.6879862
Produced by FastQC (version 0.11.2)