FastQCFastQC Report
Wed 22 Jul 2015
G88-M5_CAGATC_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M5_CAGATC_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15092239
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2701.157396E-515.5056361
CGATCAC26250.013.6367753
CTCGAAT43200.012.2752951
CCGGAAT45950.011.9455821
CGCGGAT14050.011.5878411
GAATCGC24300.011.2874553
TAAGGCG57000.011.2542659
CACGATC36850.010.9822831
ACGATCA31250.010.711142
AGATCGG103550.010.5057192-93
CGCAAAT13000.010.3768491
CGCAATT21450.010.1925161
TAAATCG6901.077733E-710.1063073
CCGGGAT32900.010.0386031
AATCGCA29750.010.0001354
TCGAATC18650.09.9708992
CTGGAAT200850.09.958811
AATCGTG19450.09.7989615
CGACGAT11450.09.7502691
CGATTGG46750.09.7444514
Produced by FastQC (version 0.11.2)