FastQCFastQC Report
Wed 22 Jul 2015
G88-M4_GCCAAT_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M4_GCCAAT_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434256
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC27500.014.1188313
GAATCGC24300.012.9442843
CGCAATT25050.012.862551
CTCGAAT43900.012.0820521
CTAGCCT79250.011.5090641
ACGATCA30750.011.4430412
CGGAAAT35050.011.1727611
CACGATC39200.010.7486591
ATTGGAC80700.010.65786656
TAGCCTC78600.010.6559542
AATCGCA33200.010.510534
CTGGAAT224650.010.4370121
CGATTGG64900.010.3748114
GGAATTG138350.010.33756353
GTTCGAT24500.010.3187131
CTCGCAT20400.010.2056471
AGATCGG124000.010.14344392-93
GCGATTG56250.010.1353743
GATCGGA107950.010.06560492-93
CGATTAG25200.09.7515544
Produced by FastQC (version 0.11.2)