FastQCFastQC Report
Wed 22 Jul 2015
G88-M4_GCCAAT_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M4_GCCAAT_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434256
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3205.7442987E-513.08252051
GAATCGC25800.012.9730873
CGATCAC25750.012.4566823
CGCGCAT10650.012.2294611
TAAGGCG63700.012.1866729
ATTGGAC79250.011.4965156
AATCGCA33600.011.2060834
CTCGAAT44900.010.8778181
CGCGTGT11400.010.6088281
CTAGCCT77050.010.5648741
ACGATCA29700.010.3304522
GATCGGA109950.010.1690692-93
AGATCGG126450.010.05540292-93
CCGGAAT52300.010.05022051
CTGGAAT228600.010.0112371
CGGGAAT32500.09.8756251
CGGAAAT35600.09.799641
GATTGGC152050.09.6607325
CGCGTTT8251.4433681E-89.5850381
CACGATC37950.09.5605241
Produced by FastQC (version 0.11.2)