FastQCFastQC Report
Wed 22 Jul 2015
G88-M3_ACAGTG_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M3_ACAGTG_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13032720
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTG16600.012.7315875
TAAGGCG39200.012.6035439
CGATCAC21800.012.1747343
ATTGGAC62750.011.5139896
CGTATCG3050.0051566911.2802943
CGGATAT19650.011.0830391
CTCGAAT34200.010.8543511
CGCTAAC5101.8973478E-510.6755741
CGGAAAT24850.010.55645751
CTCGAAC30950.010.554851
AGATCGG98500.010.43243192-93
CGATTGG58500.010.4180894
CGATAAC12000.010.3116351
ATATGCG6201.5001533E-610.30561356
CTGGAAT177250.010.3040711
GAAATCG30750.010.2295633
ACGATCA26150.010.21892
GAATCGC18350.010.1781723
GCGATTG54650.010.1627323
CGCCAAT14750.010.0669521
Produced by FastQC (version 0.11.2)