FastQCFastQC Report
Wed 22 Jul 2015
G88-M1_CGATGT_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M1_CGATGT_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9436789
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC20650.021.8861623
ACGATCA21150.021.3024352
CACGATC25700.019.6552031
ATTGGAC68100.016.2307016
AATTGGA85600.014.1750645
GAATTGG87800.013.7079794
CTCGTTT19150.013.7062171
CTGGAAT146850.013.0173871
GGAATTG99150.012.9315223
CGATTGG61500.012.30124
CGCCATT18850.011.8225631
GCGATTG59550.011.796583
CTCGATT9300.011.7152141
AGCGATT60000.011.5524752
GAATCGC12850.011.0865573
CGTCGAT7156.0754246E-911.082161
TTGGACT96250.011.0758157
CGATTTA3600.00142606110.9166484
CTCGAAT24250.010.8236721
CAGCGAT69650.010.6654851
Produced by FastQC (version 0.11.2)