FastQCFastQC Report
Wed 22 Jul 2015
G88-M1_CGATGT_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M1_CGATGT_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9436789
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC22000.023.877833
ACGATCA22000.022.6099822
CACGATC26650.020.0729561
CGCGTAC3651.5825208E-1017.8420961
CGCGTGT6150.015.1274451
ATTGGAC69050.014.3394346
CTTAGAT29900.012.9127171
GGTAATC25700.012.662052
AATTGGA87500.012.4846365
GAATTGG89500.012.2575884
ATATGCG4609.1757283E-712.1266136
AGTCTAA61600.011.9231896
GGAATTG100350.011.7667213
CTGGAAT157500.011.6366071
CGCGTAA2800.00410821311.6292241
CGAATCG3259.618874E-411.4431533
CGCCGTT4509.428255E-611.3707961
CGGTAAT4509.428255E-611.3707961
GTCTAAA64500.011.3147987
CCGGGAT19600.011.1545611
Produced by FastQC (version 0.11.2)