FastQCFastQC Report
Wed 22 Jul 2015
G88-M11_GTCCGC_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M11_GTCCGC_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716311
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT137050.12788915887192898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA22200.023.1691592
CGATCAC23850.022.6220993
CACGATC28000.018.726221
ATTGGAC99350.016.242646
GGTAATC30450.014.6179052
GAATTGG125000.014.2437784
AATTGGA123950.014.0478685
GGAATTG134400.013.6854973
CTCGAAT25100.013.4010091
CTGGAAT181450.013.3034761
GGGTAAT36350.013.06378651
CGCCATT23100.012.8481781
AAGACGT55900.012.1086543
GAATCGC15100.012.0185853
CGATTGG93800.011.7654434
CGGCGCT18600.011.7015131
CGCAATT13150.011.6610661
AATCGTG12200.011.6591465
AGTCTAA79100.011.6576215
AGACGTG60500.011.4312134
Produced by FastQC (version 0.11.2)