FastQCFastQC Report
Wed 22 Jul 2015
G88-M10_CCGTCC_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M10_CCGTCC_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15308968
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA31650.019.0240462
CGATCAC32200.018.7070333
CACGATC39750.016.515531
ATTGGAC105450.016.4868986
AATTGGA138100.013.90111355
GAATTGG139050.013.6300414
CTGGAAT231600.013.0434041
GGAATTG164200.012.7651613
CTCGATT14600.012.171041
CGCCATT32750.012.0574851
TTGGACT160800.011.0557957
CGCCAAT19000.010.911231
CGGGATT26350.010.6775661
AGTCTAA86000.010.6487925
CGCGTGT12750.010.4527761
AGATCGG107700.010.40246792-93
CCGGGAT32200.010.2706271
CGATTGG101700.010.2568934
GATCGGA95700.010.20168492-93
GAATCGC22600.010.1846273
Produced by FastQC (version 0.11.2)