FastQCFastQC Report
Wed 22 Jul 2015
G88-M10_CCGTCC_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M10_CCGTCC_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15308968
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC31700.016.5716363
ACGATCA33150.015.98702052
ATTGGAC110650.015.0402036
CACGATC39150.014.3764281
GAATTGG147850.012.7336934
AATTGGA145350.012.5369855
CGGAAAT37000.012.0688771
CGCGGAT17000.012.0392961
GGAATTG166850.011.8972853
CTGGAAT236400.011.491131
CGCGTGT12100.011.1483351
TCGGTTA15100.011.0833682
CTCGAAT35150.010.71906951
TAAGGCG36000.010.71746359
AGTCTAA85050.010.6035065
GTCGGTT37300.010.6000391
GAATCGC23750.010.5700333
AGATCGG109300.010.3786892-93
TTGGACT163800.010.3300867
CCGGAAT47400.010.3040591
Produced by FastQC (version 0.11.2)