FastQCFastQC Report
Mon 29 Jun 2015
G85_M8_GTCCGC_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M8_GTCCGC_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23996329
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA691040.2879773818737024TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT406870.16955510153240524TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG148550.040.27549732-33
CTCGTAT161900.036.55150644-45
TATGCCG165150.036.3587748-49
TCACGTC171050.034.89610730-31
AGATCGG211650.034.8712461
CGTATGC175100.034.22228646-47
GTCCGCA178550.033.742234-35
ATCTCGT175800.033.3176942-43
AGTCACG183500.032.93383428-29
CCGCACA191550.031.37890636-37
GATCGGA242200.030.5192172
ATCGGAA260100.028.5964373
CCGTCTT212050.028.31947952-53
TGCCGTC215050.027.82671250-51
CGGAAGA271100.027.505235
CGTCTGA222450.026.9601716-17
CACACGT237050.025.4964712-13
TCGGAAG309500.024.4827484
CACGTCT247650.024.38643614-15
AGAGCAC322650.023.1106539
Produced by FastQC (version 0.11.2)