FastQCFastQC Report
Mon 29 Jun 2015
G85_M6_CAGATC_L008_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M6_CAGATC_L008_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26269382
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA42600.029.9082446-47
GATCTCG81150.019.22452234-35
GAGCGTC107450.019.1715099
AGAGCGT112250.019.0148078
TCGTGTA66600.018.81650714-15
CCGTATC71950.018.6451148-49
CGTATCA49050.018.48610948-49
GTCGCCG70800.018.45537844-45
AAGAGCG131750.018.4240677
GATCGGA172450.016.3407731
GCCGTAT60050.015.48698546-47
TCGCCGT51350.015.16785944-45
ATCGGAA192850.014.7090032
CGGAAGA199100.013.7334334
CGTCGTG95750.013.69513812-13
AGCGTCG109800.013.38267410-11
TCTCGGT94600.012.9029736-37
TAGATCT113300.012.66124532-33
GTAGGGA128700.012.41083418-19
ATCTCGG102650.012.18554334-35
Produced by FastQC (version 0.11.2)