FastQCFastQC Report
Mon 29 Jun 2015
G85_M6_CAGATC_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG85_M6_CAGATC_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26269382
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC578570.22024499853098944TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT109600.030.30510542-43
TATGCCG113600.029.68959446-47
GATCGGA210100.029.4172211
CGTATGC122400.028.25634844-45
ATCGGAA229200.026.9762652
CGGAAGA237500.026.3848934
ATCTCGT126850.025.85417440-41
TCGGAAG273150.022.9583053
AGAGCAC287500.022.2654238
ACGTCTG142200.021.98706214-15
TGCCGTC161350.020.86222648-49
GCCGTCT148900.020.45208748-49
CCGTCTT168800.020.314750-51
CGTCTGA176800.019.9056816-17
TCGTATG156400.019.73583442-43
ACACGTC159900.019.713112-13
ATGCCGT155600.019.56937246-47
CACACGT182800.019.5325412-13
GCACACG164150.019.07478510-11
GAGCACA347800.018.8595929
Produced by FastQC (version 0.11.2)